LC3 trap-F (GST)

Product Description

LC3 traps were developed to isolate endogenous LC3/GABARAP proteins from mammalian cells (1). LC3 traps were engineered to contain multimeric LC3-interacting regions (LIRs) to increase their affinity for LC3/GABARAP proteins in various cell lines. LC3 traps F are based in 8 consensual LIR sequences with predominant hydrophobic amino-acids F typically found in the autophagy receptor OPTN. These LIRs are known to display affinity and specificity towards one or several LC3/GABARAP family members. LC3 traps F show binding preference towards GABARAP, GABARAP-L1 and GABARAP-L2 proteins in mammalian cells, and for LGG1 in C. elegans model. The affinity of LC3-traps was measured using microscale thermophoresis (MST) and estimated in the low nanomolar range (1).

B Molecular proposes LC3 trap F tagged with GST that can be attached to glutathione agarose affinity columns. LC3-traps can be easily use in GST-pull down assays to capture LC3/GABARAP proteins and cellular factors directly or indirectly associated to these ubiquitin family members from mammalian cells but also equivalent molecules LGG1 in C. elegans or ATG8 in S. cerevisiae models (1, 2). In addition to that, LC3-traps are incorporated in different in vitro and cell-based assays/kits developed by B Molecular that will be commercialized in the near future.


Tag: GST

Purity: > 95% by RP-HPLC and SDS-PAGE

Molecular Weight: 41,54      kDa

Physical State: Liquid

Quantity: 200 µg      

Solubility: <5 mg/mL

Concentration: Variable (3-5 mg/mL     )

Storage: -80°C, avoid freeze/thaw cycles


Pull down of GABARAP, GABARAP-L1 and GABARAP-L2 proteins and complexes associated to them from cell lines and distinct biologic models (eg. C elegans      and S. cerevisiae     ) using glutathione beads. Captured proteins can be analyzed by Western blot or Mass spectrometry with the appropriate adaptations. 

Quantitative or semiquantitative methods using different supports. 


Estimated 100-fold higher affinity than the single LIR domain

Avoid overexpression of tagged ATG8 molecules for pull downs

Replace GABARAP, GABARAP-L1 and GABARAP-L2 specific antibodies for enrichment of associated complexes

Examples of use

LC3 trap F can be used to GABARAP, GABARAP-L1 and GABARAP-L2 proteins implicated in the regulation of autophagy and      in the development of human diseases including inflammation, oncogenesis, neurodegenerative diseases and multiple infections (3). Autophagy activity can be followed in response to a stress condition or treatment. A LC3-traps step by step protocol has also been      reported (2).


1. Quinet G, Génin P, Ozturk O, Belgareh-Touzé N, Courtot L, Legouis R, Weil R, Cohen MM, Rodriguez MS (2021) Exploring selective autophagy events in multiple biologic models using LC3-Interacting Regions (LIR)-based molecular traps. Scientific Reports. In press

2. Quinet G, Ozturk O, Belgareh-Touzé N, Legouis R, Cohen MM, Rodriguez MS (2021) Capture of ATG8 family members using LC3-traps. MiMB. In press

3. Jiang, P. & Mizushima, N. Autophagy and human diseases. Cell Res 24, 69–79 (2014).